The ratio between collagenase class I and class II influences the efficient islet release from the rat pancreas.

Journal article

BACKGROUND: Previous studies indicated different roles of collagenase class I, class II and neutral protease in the enzymatic islet release from pancreatic tissue. Because no information has been available, this study was aimed to investigate the isolation efficiency of different ratios between collagenase class II and I (C-ratio) in the rat pancreas serving as model for the human pancreas without being restricted by the large variability observed in human donors. METHODS: Rat pancreata were...

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Authors: Brandhorst, H; Raemsch-Guenther, N; Raemsch, C; Friedrich, O; Huettler, S

• Journal: Transplantation
• Volume: 85
• Issue: 3
• Pages: 456-461
• Publication date: 2008-02-01
• DOI: 10.1097/tp.0b013e31816050c8
• EISSN: 1534-6080
• ISSN: 0041-1337
• Language: English
• Keywords: Islets of Langerhans; Cell Separation; Histocompatibility Antigens Class II; Animals; Chromatography, High Pressure Liquid; Male; Rats, Inbred Lew; Histocompatibility Antigens Class I; Rats; Collagenases