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Active site mutations of recombinant deacetoxycephalosporin C synthase.

Abstract:

Site-directed mutagenesis of active site residues of deacetoxycephalosporin C synthase active site residues was carried out to investigate their role in catalysis. The following mutations were made and their effects on the conversion of 2-oxoglutarate and the oxidation of penicillin N or G were assessed: M180F, G299N, G300N, Y302S, Y302F/G300A, Y302E, Y302H, and N304A. The Y302S, Y302E, and Y302H mutations reduced 2-oxoglutarate conversions and abolished (<2%) penicillin G oxidation. The Y...

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Publication status:
Published

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Publisher copy:
10.1006/bbrc.2002.6620

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Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Organic Chemistry
Journal:
Biochemical and biophysical research communications
Volume:
292
Issue:
1
Pages:
66-70
Publication date:
2002-03-05
DOI:
EISSN:
1090-2104
ISSN:
0006-291X
URN:
uuid:166cf04c-d559-464f-bf96-8e3a383cd616
Source identifiers:
32071
Local pid:
pubs:32071

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