Lifetime-identification of single molecules in aqueous solution

Journal article

Photon bursts of single rhodamine 6G and rhodamine B molecules in aqueous solution were studied by excitation with a frequency-doubled titanium: sapphire laser. Multichannel scalar traces, fluorescence correlation functions and fluorescence decays determined by time- correlated single-photon counting have been measured simultaneously. The time-resolved fluorescence signals were analyzed with a maximum likelihood estimator. With the setup described it is possible to distinguish single dye molecules of different kind via their characteristic fluorescence lifetimes of 1.79 +/- 0.33 ns for rhodamine B-zwitterion and 3.79 +/- 0.38 ns for rhodamine 6G. ©2005 Copyright SPIE - The International Society for Optical Engineering.

Authors: Zander, C; Sauer, M; Drexhage, K; Wolfrum, J; Brand, L

• Publication status: Published
• Journal: ADVANCES IN FLUORESCENCE SENSING TECHNOLOGY III
• Volume: 2980
• Pages: 107-114
• Publication date: 1997-01-01
• DOI: 10.1117/12.273566
• ISSN: 0277-786X
• Keywords: rhodamine 6G; rhodamine B; fluorescence lifetime; single-molecule detection