C-terminal Tensin-like (CTEN) is an oncogene which alters cell motility possibly through repression of E-cadherin in colorectal cancer.

Albasri, A; Seth, R; Jackson, D; Benhasouna, A; Crook, S; Nateri, A; Chapman, R; Ilyas, M

Journal article

C-terminal Tensin-like (CTEN) is an oncogene which alters cell motility possibly through repression of E-cadherin in colorectal cancer.

Abstract:: The Tensin gene family encodes proteins thought to modulate integrin function. C-terminal Tensin-like (CTEN) is a member of the Tensin gene family which lacks the N-terminus actin-binding domain. Cten is reported to have both oncogenic and tumour-suppressor functions. We investigated the role that Cten may play in colorectal cancer (CRC). By quantitative RT-PCR CTEN is up-regulated (i.e. > two-fold increase) in 62% of cell lines and 69% of tumours compared with normal mucosa, consistent with CTEN being a possible oncogene. Stable transfection of HCT116 and SW480 (CRC cell lines with low endogenous Cten expression) with a Cten expression vector gave identical results in both cell lines. Forced Cten expression did not cause change in cell numbers, although it did confer resistance to staurosporine-induced apoptosis (p < 0.005). Cten also induced epithelial-mesenchymal transition (EMT) in tumour cells accompanied by a significant increase in both cell migration (transwell migration and cell wounding assays, p < 0.001 and p < 0.05, respectively) and cell invasion (invasion through Matrigel, p < 0.001). Given the observed EMT, we investigated the levels of E-cadherin. Cten induction was associated with a reduction in E-cadherin protein expression but not levels of E-cadherin mRNA. These data suggest that CTEN is an oncogene in CRC which stimulates EMT, cell migration and invasion and may therefore have a role in tumour invasion/spread. Furthermore, Cten induction is associated with post-transcriptional repression of E-cadherin.

Publication status:: Published

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APA Style

Albasri, A., Seth, R., Jackson, D., Benhasouna, A., Crook, S., Nateri, A., Chapman, R., & Ilyas, M. (2009). C-terminal Tensin-like (CTEN) is an oncogene which alters cell motility possibly through repression of E-cadherin in colorectal cancer. Journal of Pathology, 218(1), 57–65.

MLA Style

Albasri, A, et al. “C-Terminal Tensin-like (CTEN) Is an Oncogene Which Alters Cell Motility Possibly through Repression of E-Cadherin in Colorectal Cancer.” Journal of Pathology, vol. 218, no. 1, 2009, pp. 57–65.

Chicago Style

Albasri, A, R Seth, D Jackson, et al. 2009. “C-Terminal Tensin-like (CTEN) Is an Oncogene Which Alters Cell Motility Possibly through Repression of E-Cadherin in Colorectal Cancer.” Journal of Pathology 218 (1): 57–65.
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