Isolation, characterization, and genetic analysis of monosomic, aneuploid mutants of Candida albicans.

Journal article

A white, prototrophic Candida albicans strain, heterozygous for the ADE2 gene (ade2/ADE2), was treated with the antimitotic agent methyl benzimidazole carbamate, and yielded red, adenine-requiring colonies at a rate of 4 x 10(-3), an order of magnitude higher than the spontaneous rate of Ade- colony formation. These red Ade- colonies were small, growing at approximately half the rate of the parent strain, and gave rise to large red colonies spontaneously. When the chromosomes of the small red...

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Authors: Barton, R; Gull, K

• Publication status: Published
• Journal: Molecular microbiology
• Volume: 6
• Issue: 2
• Pages: 171-177
• Publication date: 1992-01-01
• DOI: 10.1111/j.1365-2958.1992.tb01998.x
• EISSN: 1365-2958
• ISSN: 0950-382X
• Language: English
• Keywords: Candida albicans; Gene Expression; Temperature; Electrophoresis, Gel, Pulsed-Field; Mutation; Polymorphism, Restriction Fragment Length; Monosomy; Benzimidazoles; Karyotyping; Carbamates; Aneuploidy; Colony Count, Microbial; Genes, Fungal