Isolation, characterization, and genetic analysis of monosomic, aneuploid mutants of Candida albicans.

Journal article

A white, prototrophic Candida albicans strain, heterozygous for the ADE2 gene (ade2/ADE2), was treated with the antimitotic agent methyl benzimidazole carbamate, and yielded red, adenine-requiring colonies at a rate of 4 x 10(-3), an order of magnitude higher than the spontaneous rate of Ade- colony formation. These red Ade- colonies were small, growing at approximately half the rate of the parent strain, and gave rise to large red colonies spontaneously. When the chromosomes of the small red colonies were separated by pulsed-field gel electrophoresis, the band hybridizing with the ADE2 gene was diminished in staining intensity by half relative to the parent and large red-colony strains. Restriction fragment-length polymorphism analysis and auxotrophic mutant spectra after mutagenesis suggested that the small red Ade- strains were monosomic aneuploids lacking one of a pair of chromosome homologues, while the large red strains had regained a homologue, presumably via a second non-disjunction event. Parasexual genetic analysis of two of the auxotrophs isolated from a putative aneuploid suggested that both mutations were linked to the ADE2 gene. These experiments suggest that targeted chromosome loss and monosomic, aneuploid strains have the potential to extend the scope of genetic analysis in this diploid, asexual organism.

Authors: Barton, R; Gull, K

• Publication status: Published
• Journal: Molecular microbiology
• Volume: 6
• Issue: 2
• Pages: 171-177
• Publication date: 1992-01-01
• DOI: 10.1111/j.1365-2958.1992.tb01998.x
• EISSN: 1365-2958
• ISSN: 0950-382X
• Language: English
• Keywords: Karyotyping; Temperature; Carbamates; Electrophoresis, Gel, Pulsed-Field; Aneuploidy; Monosomy; Polymorphism, Restriction Fragment Length; Mutation; Benzimidazoles; Colony Count, Microbial; Candida albicans; Gene Expression; Genes, Fungal