Journal article icon

Journal article

Development of a combined air sampling and quantitative real-time PCR method for detection of Legionella spp.

Abstract:

The objective of this study was to develop and optimize the combined methods of air sampling and real time polymerase chain reaction (real-time PCR) for quantifying aerosol Legionella spp. Primers and TaqMan hydrolysis probe based on 5S rRNA gene specific for Legionella spp were used to amplify a specific DNA product of 84 bp. The impinger air sampler plus T-100 sampling pump was used to collect aerosol Legionella and as low as 10 fg of Legionella DNA per reaction could detected. Preliminary ...

Expand abstract

Actions


Authors


Sirigul, C More by this author
Wongwit, W More by this author
Phanprasit, W More by this author
Paveenkittiporn, W More by this author
Blacksell, SD More by this author
Expand authors...
Journal:
The Southeast Asian journal of tropical medicine and public health
Volume:
37
Issue:
3
Pages:
503-507
Publication date:
2006-05-05
ISSN:
0125-1562
URN:
uuid:1626a86b-56b7-4326-b715-47497a392d68
Source identifiers:
94633
Local pid:
pubs:94633

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP