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Development of a combined air sampling and quantitative real-time PCR method for detection of Legionella spp.

Abstract:

The objective of this study was to develop and optimize the combined methods of air sampling and real time polymerase chain reaction (real-time PCR) for quantifying aerosol Legionella spp. Primers and TaqMan hydrolysis probe based on 5S rRNA gene specific for Legionella spp were used to amplify a specific DNA product of 84 bp. The impinger air sampler plus T-100 sampling pump was used to collect aerosol Legionella and as low as 10 fg of Legionella DNA per reaction could detected. Preliminary ...

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Journal:
Southeast Asian journal of tropical medicine and public health
Volume:
37
Issue:
3
Pages:
503-507
Publication date:
2006-05-01
ISSN:
0125-1562
Source identifiers:
94633
Language:
English
Keywords:
Pubs id:
pubs:94633
UUID:
uuid:1626a86b-56b7-4326-b715-47497a392d68
Local pid:
pubs:94633
Deposit date:
2012-12-19

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