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Cardiac Tissue Slices: Preparation‚ Handling‚ and Successful Optical Mapping.

Abstract:

Cardiac tissue slices are becoming increasingly popular as a model system for cardiac electrophysiology and pharmacology research and development. Here, we describe in detail preparation, handling and optical mapping of trans-membrane potential and intracellular free calcium concentration transients (CaT) in ventricular tissue slices from guinea pigs and rabbits. Slices cut in the epicardium-tangential plane contained well-aligned in-slice myocardial cell strands ('fibres') in sub-epicardial ...

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Publisher copy:
10.1152/ajpheart.00556.2014

Authors


P Sarathchandra More by this author
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Journal:
American Journal of Physiology: Heart and Circulatory Physiology
Publication date:
2015
DOI:
URN:
uuid:160ce682-c167-4c5e-928e-c1e884622c82
Local pid:
cs:9289

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