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Development of a rapid, small-scale DNA repair assay for use on clinical samples.

Abstract:

Double-strand breaks (DSBs) are the most lethal form of DNA damage. They can be repaired by one of two pathways, homologous recombination and non-homologous end joining (NHEJ). A NHEJ assay has previously been reported which measures joining using cell-free extracts and a linearised plasmid as DNA substrate. This assay was designed for 3 x 10(9) cells grown in vitro and utilised radioactively labelled substrate. We have scaled down the method to use smaller cell numbers in a variety of cell l...

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Publication status:
Published

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Publisher copy:
10.1093/nar/gng083

Authors


Diggle, CP More by this author
Bentley, J More by this author
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Institution:
University of Oxford
Department:
Oxford, MSD, Oncology
Journal:
Nucleic acids research
Volume:
31
Issue:
15
Pages:
e83
Publication date:
2003-08-05
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:15fe8375-94af-494e-98a3-5efd351753bb
Source identifiers:
130908
Local pid:
pubs:130908

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