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Analysis of Tat targeting function and twin-arginine signal peptide activity in Escherichia coli.

Abstract:

The Tat system is a protein export system dedicated to the transport of folded proteins across the prokaryotic cytoplasmic membrane and the thylakoid membrane of plant chloroplasts. Proteins are targeted for export by the Tat system via N-terminal signal peptides harbouring an S-R-R-x-F-L-K 'twin-arginine' motif. In this chapter qualitative and quantitative assays for native Tat substrates in the model organism Escherichia coli are described. Genetic screening methods designed to allow the ra...

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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Sargent, F More by this author
Journal:
Methods in molecular biology (Clifton, N.J.)
Volume:
619
Pages:
191-216
Publication date:
2010
DOI:
EISSN:
1940-6029
ISSN:
1064-3745
URN:
uuid:15c746a3-0d39-4cf4-9524-96259c39a68e
Source identifiers:
100228
Local pid:
pubs:100228

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