Journal article
Dynamic calcium-mediated stress response and recovery signatures in the fungal pathogen, Candida albicans
- Abstract:
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Calcium (Ca2+) is an important second messenger for activating stress response signaling and cell adaptation in eukaryotic cells yet intracellular Ca2+-dynamics in fungi are poorly understood due to lack of effective real-time Ca2+ reporters. We engineered the GCaMP6f construct for use in the fungal pathogen, Candida albicans, and used live-cell imaging to observe both dynamic Ca2+ spiking and slower changes in non-spiking Ca2+-GCaMP signals elicited by stress or gene deletion. Short-term exposure to membrane, osmotic or oxidative stress generated immediate stress-specific responses and repeated exposure revealed differential recovery signatures. Osmotic stress caused yeast cell shrinkage and no adaptation response, where Ca2+-GCaMP spiking was inhibited by 1 M NaCl but not by 0.666 M CaCl2. Treatment with sodium dodecylsulfate (SDS) caused a spike-burst, raised the non-spiking Ca2+-GCaMP signals, and caused significant cell death, but surviving cells adapted over subsequent exposures. Treatment with 5 mM H2O2 abolished spiking and caused transient non-GCaMP-related autofluorescence, but cells adapted such that spiking returned and autofluorescence diminished on repeated exposure. Adaptation to H2O2 was dependent on Cap1, extracellular Ca2+, and calcineurin but not on its downstream target, Crz1. Ca2+-dynamics were not affected by H2O2 in the hog1Δ or yvc1Δ mutants, suggesting a pre-adapted, resistant state, possibly due to changes in membrane permeability. Live-cell imaging of Ca2+-GCaMP responses in individual cells has, therefore, revealed the dynamics of Ca2+-influx, signaling and homeostasis, and their role in the temporal stress response signatures of C. albicans.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 21.4MB, Terms of use)
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- Publisher copy:
- 10.1128/mbio.01157-23
Authors
- Funder identifier:
- https://ror.org/029chgv08
- Grant:
- WT204909/Z/16/Z
- Publisher:
- American Society for Microbiology
- Journal:
- mBio More from this journal
- Volume:
- 14
- Issue:
- 5
- Article number:
- e0115723
- Publication date:
- 2023-09-26
- Acceptance date:
- 2023-08-07
- DOI:
- EISSN:
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2150-7511
- ISSN:
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2161-2129
- Pmid:
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37750683
- Language:
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English
- Keywords:
- Pubs id:
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1536814
- Local pid:
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pubs:1536814
- Deposit date:
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2024-09-05
Terms of use
- Copyright holder:
- Giuraniuc et al.
- Copyright date:
- 2023
- Rights statement:
- © 2023 Giuraniuc et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.
- Notes:
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For the purpose of open access, the author has applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission.
- Licence:
- CC Attribution (CC BY)
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