PNA HyBeacons for analysis of human mutations related to statin-induced myopathy.

Journal article

Aminoalkyl and alkyne-tagged PNA HyBeacons have been synthesized, labeled with fluorescein via conventional amide bond or triazole formation (click chemistry) and used to detect single nucleotide polymorphisms (SNPs) implicated in statin-induced myopathy. The PNA HyBeacons gave much better mismatch/mutant discrimination than conventional DNA HyBeacons but smaller fluorescence changes on melting.

Authors: Gale, N; Kocalka, P; Mardle, C; Brown, T

• Journal: Artificial DNA, PNA and XNA
• Volume: 2
• Issue: 3
• Pages: 79-89
• Publication date: 2011-01-01
• DOI: 10.4161/adna.18179
• EISSN: 1949-0968
• ISSN: 1949-095X
• Language: English
• Keywords: Muscular Diseases; Click Chemistry; Oligonucleotide Probes; Hydroxymethylglutaryl-CoA Reductase Inhibitors; Polymorphism, Single Nucleotide; Transition Temperature; Genotyping Techniques; Mass Spectrometry; Fluorescent Dyes; Humans; Peptide Nucleic Acids; DNA Mutational Analysis