Journal article icon

Journal article

High-throughput production of human proteins for crystallization: the SGC experience.

Abstract:

Producing purified human proteins with high yield and purity remains a considerable challenge. We describe the methods utilized in the Structural Genomics Consortium (SGC) in Oxford, resulting in successful purification of 48% of human proteins attempted; of those, the structures of approximately 40% were solved by X-ray crystallography. The main driver has been the parallel processing of multiple (typically 9-20) truncated constructs of each target; modest diversity in vectors and host syste...

Expand abstract

Actions


Authors


Savitsky, Pavel More by this author
Bray, James More by this author
Cooper, Christopher D O More by this author
Marsden, Brian D More by this author
Mahajan, Pravin More by this author
Expand authors...
Journal:
Journal of structural biology
Volume:
172
Issue:
1
Pages:
3-13
Publication date:
2010-10-05
ISSN:
1095-8657
URN:
uuid:1552adbd-3bae-402a-b875-b9437eb95ca7
Local pid:
SGC:20541610

Terms of use


Metrics



If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP