Electrostatic and functional analysis of the seven-bladed WD beta-propellers

Journal article

β-propeller domains composed of WD repeats are highly ubiquitous and typically used as multi-site docking platforms to coordinate and integrate the activities of groups of proteins. Here, we have used extensive homology modelling of the WD40-repeat family of seven-bladed β-propellers coupled with subsequent structural classification and clustering of these models to define subfamilies of β-propellers with common structural, and probable, functional characteristics. We show that it is possible to assign seven-bladed WD β-propeller proteins into functionally different groups based on the information gained from homology modelling. We examine general structural diversity within the WD40-repeat family of seven-bladed β-propellers and demonstrate that seven-bladed β-propellers composed of WD-repeats are structurally distinct from other seven-bladed β-propellers. We further provide some insights into the multifunctional diversity of the seven-bladed WD β-propeller surfaces. This report once again reinforces the importance of structural data and the usefulness of homology models in functional classification.

Authors: Valeyev, N; Downing, A; Sondek, J; Deane, C

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Libertas Academica
• Journal: Evolutionary Bioinformatics
• Volume: 4
• Pages: 203-216
• Publication date: 2008-06-01
• Edition: Publisher's version
• ISSN: 1176-9343
• Language: English
• Subjects: Pharmacology; Statistics (see also social sciences); Biochemistry; Engineering & allied sciences