Journal article
Multi-site ultrasound-guided fine needle aspiration to study cells and soluble factors from human lymph nodes
- Abstract:
- Lymph nodes (LNs) are specialized secondary lymphoid tissues essential to the priming and maintenance of adaptive immune responses, including the B cell germinal center response; thus, they are central to immunity. However, the anatomically restricted and time-resolved nature of immune priming means that sampling disease-relevant human LNs requires specialized techniques. This article describes the application of ultrasound-guided fine-needle aspiration (FNA) to sample LNs, using cervical LNs of the head and neck as an exemplar. This minimally invasive technique allows collection of both immune cells and cell-free material that are relevant to both neuroimmune diseases and basic lymphatic functions. Downstream use of cellular material can include multiplexed flow cytometry, single-cell transcriptome sequencing (RNA-seq), and B cell cultures. The cell-free supernatant can be used for proteomics or other similar 'omics approaches. This unit describes collection of samples by FNA as well as processing and storage of samples for downstream assays. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Sampling of human cervical lymph nodes by ultrasound-guided fine-needle aspiration Alternate Protocol: Sampling of human lymph nodes by ultrasound-guided fine-needle aspiration with negative pressure Basic Protocol 2: Processing and storage of human lymph node samples.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Version of record, pdf, 865.8KB, Terms of use)
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- Publisher copy:
- 10.1002/cpz1.70063
Authors
+ Wellcome Trust
More from this funder
- Funder identifier:
- https://ror.org/029chgv08
- Grant:
- 227217/Z/23/Z
- 104079/Z/14/Z
+ Medical Research Council
More from this funder
- Funder identifier:
- https://ror.org/03x94j517
- Grant:
- MR/V007173/1
- MR/W024977/1
- MR/Y004450/1
+ Academy of Medical Sciences
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- Funder identifier:
- https://ror.org/00c489v88
- Grant:
- SGL027\1016
- Publisher:
- Wiley
- Journal:
- Current Protocols in Neuroscience More from this journal
- Volume:
- 4
- Issue:
- 11
- Article number:
- e70063
- Place of publication:
- United States
- Publication date:
- 2024-11-23
- DOI:
- EISSN:
-
2691-1299
- ISSN:
-
1934-8576
- Pmid:
-
39579041
- Language:
-
English
- Keywords:
- Pubs id:
-
2065464
- Local pid:
-
pubs:2065464
- Deposit date:
-
2024-12-09
Terms of use
- Copyright holder:
- Al-Diwani et al
- Copyright date:
- 2024
- Rights statement:
- © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Licence:
- CC Attribution (CC BY)
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