Journal article

Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis.

Abstract:: DNA threading the needle: A new method of single-molecule detection of 5-hydroxymethylcytosine (5hmC) in DNA has been developed. Selective thiol substitution of 5hmC (giving SMC) in a single-step, bisulfite-mediated reaction (see scheme) allows the incorporation of a peptide (yellow sphere) or biotin into DNA. Modified 5hmC bases can be readily distinguished at the single-molecule level using protein nanopore analysis. Copyright © 2013 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim.

Publication status:: Published

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APA Style

Li, W., Gong, L., & Bayley, J. (2013). Single-molecule detection of 5-hydroxymethylcytosine in DNA through chemical modification and nanopore analysis. Angewandte Chemie (International Ed. in English), 52(16), 4350–4355.

MLA Style

Li, W, et al. “Single-Molecule Detection of 5-Hydroxymethylcytosine in DNA through Chemical Modification and Nanopore Analysis.” Angewandte Chemie (International Ed. in English), vol. 52, no. 16, 2013, pp. 4350–55.

Chicago Style

Li, W, L Gong, and J Bayley. 2013. “Single-Molecule Detection of 5-Hydroxymethylcytosine in DNA through Chemical Modification and Nanopore Analysis.” Angewandte Chemie (International Ed. in English) 52 (16): 4350–55.
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Publisher copy:: 10.1002/anie.201300413

Authors

+ Li, W More by this author

Role:: Author

+ Gong, L More by this author

Role:: Author

+ Bayley, J More by this author

Institution:: University of Oxford
Division:: MPLS
Department:: Chemistry
Sub department:: Chemical Biology
Role:: Author

Journal:: Angewandte Chemie (International ed. in English) More from this journal
Volume:: 52
Issue:: 16
Pages:: 4350-4355
Publication date:: 2013-04-01
DOI:: 10.1002/anie.201300413
EISSN:: 1521-3773
ISSN:: 1433-7851

Language:: English
Keywords:: DNA

Cytosine

Molecular Structure

Nanopores
Pubs id:: pubs:394905
UUID:: uuid:150b331e-e352-4c23-a27d-90a34e75e077
Local pid:: pubs:394905
Source identifiers:: 394905
Deposit date:: 2013-11-17
ARK identifier:: ark:/29072/ora_150b331ee3524c23a27d90a34e75e077

Terms of use

Copyright date:: 2013

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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