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High avidity antigen-specific CTL identified by CD8-independent tetramer staining.

Abstract:

Tetrameric MHC/peptide complexes are important tools for enumerating, phenotyping, and rapidly cloning Ag-specific T cells. It remains however unclear whether they can reliably distinguish between high and low avidity T cell clones. In this report, tetramers with mutated CD8 binding site selectively stain higher avidity human and murine CTL capable of recognizing physiological levels of Ag. Furthermore, we demonstrate that CD8 binding significantly enhances the avidity as well as the stabilit...

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Publication status:
Published

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Journal:
Journal of immunology (Baltimore, Md. : 1950)
Volume:
171
Issue:
10
Pages:
5116-5123
Publication date:
2003-11-05
DOI:
EISSN:
1550-6606
ISSN:
0022-1767
URN:
uuid:14f14db5-2b5c-4cdb-b064-98d1c7f6baa4
Source identifiers:
15334
Local pid:
pubs:15334

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