High avidity antigen-specific CTL identified by CD8-independent tetramer staining.

Journal article

Tetrameric MHC/peptide complexes are important tools for enumerating, phenotyping, and rapidly cloning Ag-specific T cells. It remains however unclear whether they can reliably distinguish between high and low avidity T cell clones. In this report, tetramers with mutated CD8 binding site selectively stain higher avidity human and murine CTL capable of recognizing physiological levels of Ag. Furthermore, we demonstrate that CD8 binding significantly enhances the avidity as well as the stabilit...

Expand abstract

Authors: Choi, E; Chen, J; Wooldridge, L; Salio, M; Lissina, A

• Publication status: Published
• Journal: Journal of Immunology
• Volume: 171
• Issue: 10
• Pages: 5116-5123
• Publication date: 2003-11-01
• DOI: 10.4049/jimmunol.171.10.5116
• EISSN: 1550-6606
• ISSN: 0022-1767
• Language: English
• Keywords: Humans; Antigens, Neoplasm; Plasmids; Cytotoxicity, Immunologic; Epitopes, T-Lymphocyte; beta 2-Microglobulin; Membrane Proteins; Vaccinia; Staining and Labeling; Cell Line; Antigens, CD8; Mice; Lymphocyte Activation; Animals; Immunization, Secondary; Clone Cells; Cell Line, Tumor; HLA-A2 Antigen; H-2 Antigens; Jurkat Cells; T-Lymphocytes, Cytotoxic; Proteins; Mice, Transgenic; Binding Sites