Voltammetric studies of the reactions of iron-sulphur clusters ([3Fe-4S] or [M3Fe-4S]) formed in Pyrococcus furiosus ferredoxin.

Journal article

Reactions of the [3Fe-4S] cluster and various metallated [M3Fe-4S] adducts co-ordinated in the ferredoxin from the hyperthermophile Pyrococcus furiosus have been studied by protein-film voltammetry, bulk-solution voltammetry, solution kinetics and magnetic CD (MCD). The [3Fe-4S] cluster exhibits two couples, [3Fe-4S]+/0 and [3Fe-4S]0/2-. Film voltammetry is possible over a wide pH range (2-8), revealing that the [3Fe-4S]+/0 couple shows a complex pH dependence with pKred1=2.8, pKox=4.9 and pK...

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Authors: Fawcett, SE; Davis, D; Breton, J; Thomson, A; Armstrong, F

• Publication status: Published
• Journal: Biochemical journal
• Volume: 335 ( Pt 2)
• Issue: 2
• Pages: 357-368
• Publication date: 1998-10-01
• EISSN: 1470-8728
• ISSN: 0264-6021
• Language: English
• Keywords: Electrophysiology; Spectrum Analysis; Iron; Hydrogen-Ion Concentration; Sulfur; Spectrophotometry, Ultraviolet; Electric Conductivity; Kinetics; Ferredoxins; Magnetics; Oxidation-Reduction; Metals; Pyrococcus furiosus; Bacterial Proteins; Thermodynamics