RNA polymerase II kinetics in polo polyadenylation signal selection.

Journal article

Regulated alternative polyadenylation is an important feature of gene expression, but how gene transcription rate affects this process remains to be investigated. polo is a cell-cycle gene that uses two poly(A) signals in the 3' untranslated region (UTR) to produce alternative messenger RNAs that differ in their 3'UTR length. Using a mutant Drosophila strain that has a lower transcriptional elongation rate, we show that transcription kinetics can determine alternative poly(A) site selection. ...

Expand abstract

Authors: Pinto, P; Henriques, T; Freitas, M; Martins, T; Domingues, R

• Publication status: Published
• Journal: EMBO journal
• Volume: 30
• Issue: 12
• Pages: 2431-2444
• Publication date: 2011-06-01
• DOI: 10.1038/emboj.2011.156
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: Kinetics; Cell Proliferation; Poly A; Animals; Drosophila Proteins; Polyadenylation; Signal Transduction; Drosophila melanogaster; Cell Survival; Protein-Serine-Threonine Kinases; 3' Untranslated Regions; Genetic Variation; RNA Polymerase II; Animals, Genetically Modified