Genetic analysis of the dihydrofolate reductase-thymidylate synthase gene from geographically diverse isolates of Plasmodium malariae.

Journal article

Plasmodium malariae, the parasite responsible for quartan malaria, is transmitted in most areas of malaria endemicity and is associated with significant morbidity. The sequence of the gene coding for the enzyme dihydrofolate reductase-thymidylate synthase (DHFR-TS) was obtained from field isolates of P. malariae and from the closely related simian parasite Plasmodium brasilianum. The two sequences were nearly 100% homologous, adding weight to the notion that they represent genetically distinc...

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Authors: Tanomsing, N; Imwong, M; Pukrittayakamee, S; Chotivanich, K; Looareesuwan, S

• Publication status: Published
• Journal: Antimicrobial agents and chemotherapy
• Volume: 51
• Issue: 10
• Pages: 3523-3530
• Publication date: 2007-10-01
• DOI: 10.1128/aac.00234-07
• EISSN: 1098-6596
• ISSN: 0066-4804
• Language: English
• Keywords: Polymorphism, Genetic; Molecular Sequence Data; Tetrahydrofolate Dehydrogenase; Parasitic Sensitivity Tests; Reverse Transcriptase Polymerase Chain Reaction; Antimalarials; Mutation; Thymidylate Synthase; Plasmodium malariae; Asia; DNA Primers; DNA, Protozoan; Amino Acid Sequence; Malaria; Phylogeny; Cloning, Molecular; Animals; Humans