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Transcriptomics of Diphyllatea ( CRuMs ) from South Pacific crater lakes confirm new cryptic clades

Abstract:
The Diphyllatea (CRuMs) are heterotrophic protists currently divided into three distinct clades (Diphy I–III). Diphy I are biflagellates in the genus Diphylleia, whereas Diphy II and III represent cryptic clades comprising Collodictyon‐type quadriflagellates that were recently distinguished based on rRNA gene phylogenies. Here, we isolated Diphyllatea from freshwater crater lakes on two South Pacific islands and generated high‐quality transcriptomes from species representing each clade, including the first transcriptomic data from Diphy III. Phylogenomic analyses support the separation of Diphy II and III, while transcriptome completeness highlights the utility of these data for future studies. Lastly, we discuss the biogeography and ecology of Diphyllatea on these remote islands.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1111/jeu.13060

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Institution:
University of Oxford
Role:
Author
ORCID:
0000-0003-3642-3200
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Institution:
University of Oxford
Role:
Author
ORCID:
0000-0002-7695-034X
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Institution:
University of Oxford
Role:
Author
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Role:
Author
ORCID:
0000-0002-6534-4758
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Institution:
University of Oxford
Role:
Author
ORCID:
0000-0002-9692-0973


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Funder identifier:
https://ror.org/03wnrjx87
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Funder identifier:
https://ror.org/04wfr2810


Publisher:
Wiley
Journal:
The Journal of Eukaryotic Microbiology More from this journal
Article number:
e13060
Publication date:
2024-09-28
Acceptance date:
2024-09-12
DOI:
EISSN:
1550-7408
ISSN:
1550-7408, 1066-5234


Language:
English
Keywords:
Pubs id:
2037851
Local pid:
pubs:2037851
Source identifiers:
2292628
Deposit date:
2024-09-28
ARK identifier:
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