Journal article
A new architecture for DNA-templated synthesis in which abasic sites protect reactants from degradation
- Abstract:
- The synthesis of artificial sequence-defined polymers that match and extend the functionality of proteins is an important goal in materials science. One way of achieving this is to program a sequence of chemical reactions between precursor building blocks by means of attached oligonucleotide adapters. However, hydrolysis of the reactive building blocks has so far limited the length and yield of product that can be obtained using DNA-templated reactions. Here, we report an architecture for DNA-templated synthesis in which reactants are tethered at internal abasic sites on opposite strands of a DNA duplex. We show that an abasic site within a DNA duplex can protect a nearby thioester from degradation, significantly increasing the yield of a DNA-templated reaction. This protective effect has the potential to overcome the challenges associated with programmable sequence-controlled synthesis of long non-natural polymers by extending the lifetime of the reactive building blocks.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 1.8MB, Terms of use)
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(Preview, Supplementary materials, pdf, 1.7MB, Terms of use)
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- Publisher copy:
- 10.1002/anie.202317482
Authors
- Publisher:
- Wiley
- Journal:
- Angewandte Chemie International Edition More from this journal
- Volume:
- 63
- Issue:
- 14
- Article number:
- e202317482
- Publication date:
- 2024-02-29
- Acceptance date:
- 2024-02-07
- DOI:
- EISSN:
-
1521-3773
- ISSN:
-
1433-7851
- Language:
-
English
- Keywords:
- Pubs id:
-
1616457
- Local pid:
-
pubs:1616457
- Deposit date:
-
2024-02-13
Terms of use
- Copyright holder:
- Frommer et al.
- Copyright date:
- 2024
- Rights statement:
- © 2024 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- Notes:
- For the purpose of Open Access, the authors have applied a CC BY public copyright license to any Author Accepted Manuscript version arising from this submission.
- Licence:
- CC Attribution (CC BY)
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