Journal article
Time-resolved fluorescence measurements using stroboscopic excitation
- Abstract:
-
We report on the development of a simple technique for obtaining time-domain information using dc detection of fluorescence. We show that this is feasible for assays where a change in lifetime of an indicator occurs in reaction to an analyte, in fluorescence resonance energy transfer for example, and could be particularly useful for assays performed in the scaled-down environment of a "lab-on-a-chip", A rate equation model is presented which allows an objective analysis of the relative import...
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Bibliographic Details
- Journal:
- Progress in Biomedical Optics and Imaging - Proceedings of SPIE
- Volume:
- 5864
- Pages:
- 1-7
- Publication date:
- 2005-01-01
- ISSN:
-
1605-7422
Item Description
- Language:
- English
- Pubs id:
-
pubs:132766
- UUID:
-
uuid:14429a75-de10-4db9-9a05-d6d34d9796ac
- Local pid:
- pubs:132766
- Source identifiers:
-
132766
- Deposit date:
- 2012-12-19
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- Copyright date:
- 2005
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