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Zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC and ZIC-cHILIC) provide high resolution separation and increase sensitivity in proteome analysis.

Abstract:

The complexity of peptide mixtures that are analyzed in proteomics necessitates fractionation by multidimensional separation approaches prior to mass spectrometric analysis. In this work, we introduce and evaluate hydrophilic interaction liquid chromatography (HILIC) based strategies for the separation of complex peptide mixtures. The two zwitterionic HILIC materials (ZIC-HILIC and ZIC-cHILIC) chosen for this work differ in the spatial orientation of the positive and negative charged groups. ...

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Publication status:
Published

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Publisher copy:
10.1021/ac103312e

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Institution:
University of Oxford
Division:
MPLS
Department:
Chemistry
Sub department:
Physical & Theoretical Chem
Role:
Author
Journal:
Analytical chemistry
Volume:
83
Issue:
9
Pages:
3440-3447
Publication date:
2011-05-01
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
Source identifiers:
384570
Language:
English
Keywords:
Pubs id:
pubs:384570
UUID:
uuid:1407a75c-08ee-46fa-9fe8-7d956beb02dc
Local pid:
pubs:384570
Deposit date:
2013-11-16

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