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Zwitterionic hydrophilic interaction liquid chromatography (ZIC-HILIC and ZIC-cHILIC) provide high resolution separation and increase sensitivity in proteome analysis.

Abstract:

The complexity of peptide mixtures that are analyzed in proteomics necessitates fractionation by multidimensional separation approaches prior to mass spectrometric analysis. In this work, we introduce and evaluate hydrophilic interaction liquid chromatography (HILIC) based strategies for the separation of complex peptide mixtures. The two zwitterionic HILIC materials (ZIC-HILIC and ZIC-cHILIC) chosen for this work differ in the spatial orientation of the positive and negative charged groups. ...

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Publication status:
Published

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Publisher copy:
10.1021/ac103312e

Authors


Di Palma, S More by this author
Boersema, PJ More by this author
More by this author
Institution:
University of Oxford
Department:
Oxford, MPLS, Chemistry, Physical and Theoretical Chem
Journal:
Analytical chemistry
Volume:
83
Issue:
9
Pages:
3440-3447
Publication date:
2011-05-05
DOI:
EISSN:
1520-6882
ISSN:
0003-2700
URN:
uuid:1407a75c-08ee-46fa-9fe8-7d956beb02dc
Source identifiers:
384570
Local pid:
pubs:384570

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