Field evaluation of a novel colorimetric method--double-site enzyme-linked lactate dehydrogenase immunodetection assay--to determine drug susceptibilities of Plasmodium falciparum clinical isolates from northwestern Thailand.

Journal article

A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/microl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.

Authors: Brockman, A; Singlam, S; Phiaphun, L; Looareesuwan, S; White, N

• Publication status: Published
• Journal: Antimicrobial agents and chemotherapy
• Volume: 48
• Issue: 4
• Pages: 1426-1429
• Publication date: 2004-04-01
• DOI: 10.1128/aac.48.4.1426-1429.2004
• EISSN: 1098-6596
• ISSN: 0066-4804
• Language: English
• Keywords: Malaria, Falciparum; Thailand; Plasmodium falciparum; Erythrocytes; Antimalarials; L-Lactate Dehydrogenase; Enzyme-Linked Immunosorbent Assay; Humans; Colorimetry; Nonlinear Dynamics; Animals