Quantification of membrane protein inhibition by optical ion flux in a droplet interface bilayer array.

Journal article

Abstract:: Optical platforms for assaying membrane protein function offer a promising route to scalable high-throughput screening (see picture). For the first time quantitative measurements of membrane protein inhibition are reported in an optically addressable lipid bilayer array. Wide-field total internal reflection fluorescence (TIRF) imaging of Ca 2+ flux enables the quantification of α-hemolysin inhibition by γ-cyclodextrin. Copyright © 2012 WILEY-VCH Verlag GmbH and Co. KGaA, Weinheim.

Journal:: Angewandte Chemie (International ed. in English) More from this journal
Volume:: 51
Issue:: 13
Pages:: 3134-3138
Publication date:: 2012-03-01
DOI:: 10.1002/anie.201107343
EISSN:: 1521-3773
ISSN:: 1433-7851

Language:: English
Keywords:: Protein Array Analysis

gamma-Cyclodextrins

Microscopy, Fluorescence

High-Throughput Screening Assays

Calcium

Equipment Design

Bacterial Toxins

Staphylococcus aureus

Hemolysin Proteins

Lipid Bilayers
Pubs id:: pubs:254911
UUID:: uuid:127c1472-1ad6-444c-a5ef-7c789d34229a
Local pid:: pubs:254911
Source identifiers:: 254911
Deposit date:: 2012-12-19
ARK identifier:: ark:/29072/ora_127c14721ad6444ca5ef7c789d34229a

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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