Biophysical Studies of Oligonucleotides Containing Duplex Stabilising Modifications

Thesis

Chemically modified DNA and RNA probes have been used in numerous diagnostic (e.g. fluorogenic PCR and in situ hybridisation) and therapeutic (e.g. exon-skipping and antisense) applications to increase duplex stability and facilitate mismatch discrimination. There is a constant need for well-studied modifications, and to this end X-pyrene was evaluated as a new cytosine analogue that combines enhanced π-stacking, hydrogen bonding and electrostatic interactions to greatly increase ...

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Authors: Marafini, P

• Type of award: DPhil
• Level of award: Doctoral
• Awarding institution: University of Oxford
• Language: English
• Keywords: DNA; Nucleic Acids; RNA; Crystallography; Labelling of Oligonucleotides; Pyrene
• Subjects: Chemistry; Biochemistry; Chemical Biology; Biophysics