SRβ coordinates signal sequence release from SRP with ribosome binding to the translocon

Journal article

Protein targeting to the endoplasmic reticulum (ER) membrane is regulated by three GTPases, the 54 kDa subunit of the signal recognition particle (SRP) and the α- and β-subunits of the SRP receptor (SR). Using a soluble form of SR and an XTP-binding mutant of SRβ, we show that SRβ is essential for protein translocation across the ER membrane. SRβ can be cross-linked to a 21 kDa ribosomal protein in its empty and GDP-bound state, but not when GTP is bound. GTP binding to SRβ is required to ind...

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Authors: Fulga, T; Sinning, I; Dobberstein, B; Pool, MR

• Journal: EMBO Journal
• Volume: 20
• Issue: 9
• Pages: 2338-2347
• Publication date: 2001-05-01
• DOI: 10.1093/emboj/20.9.2338
• ISSN: 0261-4189
• Language: English
• Keywords: Signal recognition particle receptor (SR); GTPase; Endoplasmic reticulum; Ribosome; Protein translocation