An oligo-screening strategy to fill gaps found during shotgun sequencing projects.

Journal article

Abstract:: During the course of projects to sequence human and nematode cosmids we encountered great difficulties generating contiguous sequence in regions with repetitive DNA (Alu repeats in humans and tandem or inverted repeats in the nematode). We have developed a simple and efficient strategy to fill gaps. By screening M13, plasmid or phagemid libraries with oligonucleotides flanking the gap, clones are identified that contiguate the cosmid sequence. Our method has been integrated into the GAP4 sequence assembly program. The strategy reduces both time and costs in large scale sequencing projects.

Journal:: DNA sequence : the journal of DNA sequencing and mapping More from this journal
Volume:: 8
Issue:: 4
Pages:: 241-245
Publication date:: 1998-03-01
EISSN:: 1029-2365
ISSN:: 1042-5179

Language:: English
Keywords:: Humans

Sequence Analysis, DNA

Cosmids

Oligodeoxyribonucleotides

Software

Nematoda

Animals

Genome

Gene Library
Pubs id:: pubs:31563
UUID:: uuid:1225b1fb-b44a-4358-a252-c907c37ec551
Local pid:: pubs:31563
Source identifiers:: 31563
Deposit date:: 2012-12-19
ARK identifier:: ark:/29072/ora_1225b1fbb44a4358a252c907c37ec551

Licence:: Terms and Conditions of Use for Oxford University Research Archive

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