Crystallization and X-ray diffraction analysis of the DNA-remodelling protein DnaD from Bacillus subtilis.

Journal article

The DnaD protein is an essential component of the chromosome-replication machinery of the Gram-positive bacterium Bacillus subtilis and is part of the primosomal cascade that ultimately loads the replicative ring helicase DnaC onto DNA. Moreover, DnaD is a global regulator of DNA architecture, as it forms higher order nucleoprotein structures in order to open supercoiled DNA. Here, the crystallization and preliminary X-ray diffraction analysis of the two domains of DnaD from B. subtilis are r...

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Authors: Schneider, S; Carneiro, M; Ioannou, C; Soultanas, P; Paoli, M

• Publication status: Published
• Journal: Acta crystallographica. Section F, Structural biology and crystallization communications
• Volume: 63
• Issue: Pt 2
• Pages: 110-113
• Publication date: 2007-02-01
• DOI: 10.1107/s1744309107000474
• EISSN: 1744-3091
• ISSN: 1744-3091
• Language: English
• Keywords: Bacillus subtilis; Bacterial Proteins; Cloning, Molecular; Crystallography, X-Ray; Crystallization; Escherichia coli; DNA-Binding Proteins