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Spin-label studies of lipid-protein interactions with reconstituted band 3, the human erythrocyte chloride-bicarbonate exchanger.

Abstract:

Lipid-protein interactions in reconstituted band 3 preparations were investigated by using spin-labeled lipids in conjunction with electron paramagnetic resonance (EPR) spectroscopy. Purified erythrocyte band 3 was reconstituted into egg phosphatidylcholine liposomes at high protein density with preservation predominantly of the dimeric state. Lipid-protein associations were revealed by the presence of a component in the EPR spectra that, when compared to spectra obtained from protein-free bi...

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Publication status:
Published

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Publisher copy:
10.1139/bcb-76-5-815

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Institution:
University of Oxford
Department:
Oxford, MSD, Biochemistry
Role:
Author
Journal:
Biochemistry and cell biology = Biochimie et biologie cellulaire
Volume:
76
Issue:
5
Pages:
815-822
Publication date:
1998-01-01
DOI:
EISSN:
1208-6002
ISSN:
0829-8211
URN:
uuid:1218e190-4be8-4abe-a77a-a5c6a9847cc8
Source identifiers:
410480
Local pid:
pubs:410480

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