Comparative protein profiling of serum and plasma using an antibody suspension bead array approach.

Journal article

In the pursuit towards a systematic analysis of human diseases, array-based approaches within antibody proteomics offer high-throughput strategies to discover protein biomarkers in serum and plasma. To investigate the influence of sample preparation on such discovery attempts, we report on a systematic effort to compare serum and plasma protein profiles determined with an antibody suspension bead array. The intensity levels were used to define protein profiles and no significant differences b...

Expand abstract

Authors: Schwenk, J; Igel, U; Kato, B; Nicholson, G; Karpe, F

• Publication status: Published
• Journal: Proteomics
• Volume: 10
• Issue: 3
• Pages: 532-540
• Publication date: 2010-02-01
• DOI: 10.1002/pmic.200900657
• EISSN: 1615-9861
• ISSN: 1615-9853
• Language: English
• Keywords: Protein Array Analysis; Biological Markers; Antibodies; Validation Studies as Topic; Cluster Analysis; Humans; Proteomics; Metabolic Syndrome X; Thrombospondins; Antibody Specificity; Blood Proteins; Cohort Studies; Biotinylation