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Journal article

Expression, purification, crystallization and preliminary crystallographic analysis of BipD, a component of the Burkholderia pseudomallei type III secretion system.

Abstract:

A construct consisting of residues 10-310 of BipD, a component of the Burkholderia pseudomallei type III secretion system (T3SS), has been overexpressed as a GST fusion, cleaved from the GST tag and purified. Crystals were grown of native and selenomethionine-labelled BipD. The crystals grow in two different polymorphs from the same condition. The first polymorph belongs to space group C222, with unit-cell parameters a = 103.98, b = 122.79, c = 49.17 A, a calculated Matthews coefficient of 2....

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Publication status:
Published

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Publisher copy:
10.1107/s1744309106027035

Authors


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Institution:
University of Oxford
Division:
MSD
Department:
Pathology Dunn School
Role:
Author
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Journal:
Acta crystallographica. Section F, Structural biology and crystallization communications
Volume:
62
Issue:
Pt 9
Pages:
861-864
Publication date:
2006-09-01
DOI:
EISSN:
1744-3091
ISSN:
1744-3091
Source identifiers:
29361
Language:
English
Keywords:
Pubs id:
pubs:29361
UUID:
uuid:11acf923-f266-40ad-9068-8dd5ac8fc2f5
Local pid:
pubs:29361
Deposit date:
2012-12-19

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