Ribotyping and DNA macrorestriction analysis of isolates of Burkholderia pseudomallei from cases of melioidosis in Malaysia.

Journal article

Forty-nine isolates of Burkholderia pseudomallei from sporadic cases of melioidosis in Malaysia over the past 18 years were examined by BamHI ribotyping and pulsed-field gel electrophoresis (PFGE) of XbaI digests of total deoxyribonucleic acid (DNA). Twenty-four patients had septicaemic melioidosis with a mortality of 70%; mortality in the non-septicaemic disease was 16%. Five ribotype patterns were identified, 2 of which accounted for 90% of all isolates. PFGE revealed a number of different strains within these ribotypes, but some pairs of isolates from unrelated cases gave closely similar DNA profiles. These results are in agreement with Australian studies which showed a high prevalence of a few ribotypes of B. pseudomallei which are further divisible by genotyping, in areas where melioidosis is endemic.

Authors: Vadivelu, J; Puthucheary, S; Mifsud, A; Drasar, B; Dance, D

• Publication status: Published
• Journal: Transactions of the Royal Society of Tropical Medicine and Hygiene
• Volume: 91
• Issue: 3
• Pages: 358-360
• Publication date: 1997-01-01
• DOI: 10.1016/s0035-9203(97)90107-3
• EISSN: 1878-3503
• ISSN: 0035-9203
• Language: English
• Keywords: Electrophoresis, Gel, Pulsed-Field; Burkholderia pseudomallei; Middle Aged; Adult; Aged; Melioidosis; Deoxyribonuclease BamHI; DNA, Ribosomal; Male; Female; Humans; Malaysia; DNA, Bacterial; Restriction Mapping