The disappearance of cyclin B at the end of mitosis is regulated spatially in Drosophila cells.

Journal article

We have followed the behaviour of a cyclin B-green fluorescent protein (GFP) fusion protein in living Drosophila embryos in order to study how the localization and destruction of cyclin B is regulated in space and time. We show that the fusion protein accumulates at centrosomes in interphase, in the nucleus in prophase, on the mitotic spindle in prometaphase and on the microtubules that overlap in the middle of the spindle in metaphase. In cellularized embryos, toward the end of metaphase, th...

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Authors: Huang, J; Raff, J

• Publication status: Published
• Journal: EMBO journal
• Volume: 18
• Issue: 8
• Pages: 2184-2195
• Publication date: 1999-04-01
• DOI: 10.1093/emboj/18.8.2184
• EISSN: 1460-2075
• ISSN: 0261-4189
• Language: English
• Keywords: Cyclin B; Mitotic Spindle Apparatus; Drosophila; Larva; Luminescent Proteins; Green Fluorescent Proteins; Recombinant Fusion Proteins; Mitosis; Kinetics; Drosophila Proteins; Animals