Journal article
T7 RNA polymerase functions in vitro without clustering.
- Abstract:
- Many nucleic acid polymerases function in clusters known as factories. We investigate whether the RNA polymerase (RNAP) of phage T7 also clusters when active. Using 'pulldowns' and fluorescence correlation spectroscopy we find that elongation complexes do not interact in vitro with a K(d)<1 micrometre. Chromosome conformation capture also reveals that genes located 100 kb apart on the E. coli chromosome do not associate more frequently when transcribed by T7 RNAP. We conclude that if clustering does occur in vivo, it must be driven by weak interactions, or mediated by a phage-encoded protein.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Preview, Version of record, pdf, 389.4KB, Terms of use)
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- Publisher copy:
- 10.1371/journal.pone.0040207
Authors
+ Biotechnology and Biological Sciences Research Council
More from this funder
- Funding agency for:
- Kapanidis, A
- Grant:
- BB/H01795X/1
+ Clarendon Fund, University of Oxford
More from this funder
- Funding agency for:
- Finan, K
- Torella, J
- Publisher:
- Public Library of Science
- Journal:
- PloS one More from this journal
- Volume:
- 7
- Issue:
- 7
- Article number:
- e40207
- Publication date:
- 2012-01-01
- DOI:
- EISSN:
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1932-6203
- ISSN:
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1932-6203
- Language:
-
English
- Keywords:
- UUID:
-
uuid:11113cc0-25b9-48c1-8713-5d4d12133986
- Local pid:
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pubs:342278
- Source identifiers:
-
342278
- Deposit date:
-
2012-12-19
Terms of use
- Copyright holder:
- Scholl et al
- Copyright date:
- 2012
- Notes:
- Copyright 2012 Scholl et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Licence:
- CC Attribution (CC BY)
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