Wrestling with native chemical ligation.

Journal article

An improved method for the semisynthesis of a potassium channel involving native chemical ligation allows the introduction of short sequences containing non-canonical amino acids at any position within the polypeptide chain. The work enhances the technology available for a range of fundamental investigations of membrane proteins and for applications of membrane channels and pores in biotechnology.

Authors: Bayley, H; Cheley, S; Harrington, L; Syeda, R

• Publication status: Published
• Journal: ACS chemical biology
• Volume: 4
• Issue: 12
• Pages: 983-985
• Publication date: 2009-12-01
• DOI: 10.1021/cb900304p
• EISSN: 1554-8937
• ISSN: 1554-8929
• Language: English
• Keywords: Models, Molecular; Amino Acid Sequence; Rats; Amino Acids; Bacterial Proteins; Potassium Channels; Protein Conformation; Animals