Journal article
Dimensions and global twist of single-layer DNA origami measured by small-angle X-ray scattering
- Abstract:
- The rational design of complementary DNA sequences can be used to create nanostructures that self-assemble with nanometer precision. DNA nanostructures have been imaged by atomic force microscopy and electron microscopy. Small-angle X-ray scattering (SAXS) provides complementary structural information on the ensemble-averaged state of DNA nanostructures in solution. Here we demonstrate that SAXS can distinguish between different single-layer DNA origami tiles that look identical when immobilized on a mica surface and imaged with atomic force microscopy. We use SAXS to quantify the magnitude of global twist of DNA origami tiles with different crossover periodicities: these measurements highlight the extreme structural sensitivity of single-layer origami to the location of strand crossovers. We also use SAXS to quantify the distance between pairs of gold nanoparticles tethered to specific locations on a DNA origami tile and use this method to measure the overall dimensions and geometry of the DNA nanostructure in solution. Finally, we use indirect Fourier methods, which have long been used for the interpretation of SAXS data from biomolecules, to measure the distance between DNA helix pairs in a DNA origami nanotube. Together, these results provide important methodological advances in the use of SAXS to analyze DNA nanostructures in solution and insights into the structures of single-layer DNA origami.
- Publication status:
- Published
- Peer review status:
- Peer reviewed
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- Files:
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(Preview, Accepted manuscript, pdf, 1.7MB, Terms of use)
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- Publisher copy:
- 10.1021/acsnano.8b01669
Authors
+ Human Frontiers Science Program
More from this funder
- Funding agency for:
- Turberfield, A
- Bath, J
- Lee, L
- Grant:
- WM110130
- RGP0030/2013
- RGP0030/2013
- Publisher:
- American Chemical Society
- Journal:
- ACS Nano More from this journal
- Volume:
- 12
- Issue:
- 6
- Pages:
- 5791-5799
- Publication date:
- 2018-05-29
- Acceptance date:
- 2018-05-29
- DOI:
- EISSN:
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1936-086X
- ISSN:
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1936-0851
- Pmid:
-
29812934
- Language:
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English
- Keywords:
- Pubs id:
-
pubs:854568
- UUID:
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uuid:10a3e3c2-0fc0-4b63-b0ad-1cdd6d10c625
- Local pid:
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pubs:854568
- Source identifiers:
-
854568
- Deposit date:
-
2018-07-25
Terms of use
- Copyright holder:
- © 2018 American Chemical Society
- Copyright date:
- 2018
- Notes:
- This is the accepted manuscript version of the article. The final version is available online from the American Chemical Society at: 10.1021/acsnano.8b01669
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