Fluorescent cyclic voltammetry of immobilized azurin: direct observation of thermodynamic and kinetic heterogeneity.
(Figure Presented) Variations in the formal electrochemical potential (E 0) and electron-transfer rates (K 0) of the blue copper protein azurin have been directly observed. A new method, fluorescent cyclic voltammetry (FCV), was used to resolve the properties of 100-1000 proteins. On this scale, the presence of large variations in the values of both E 0 and k 0 could be established and several form...Expand abstract
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