Fluorescent cyclic voltammetry of immobilized azurin: direct observation of thermodynamic and kinetic heterogeneity.

Journal article

(Figure Presented) Variations in the formal electrochemical potential (E 0) and electron-transfer rates (K 0) of the blue copper protein azurin have been directly observed. A new method, fluorescent cyclic voltammetry (FCV), was used to resolve the properties of 100-1000 proteins. On this scale, the presence of large variations in the values of both E 0 and k 0 could be established and several forms of heterogeneity were differentiated. © 2010 Wiley-VCH Verlag GmbH and Co. KGaA, Weinheim.

Authors: Salverda, J; Patil, A; Mizzon, G; Kuznetsova, S; Zauner, G

• Publication status: Published
• Journal: Angewandte Chemie (International ed. in English)
• Volume: 49
• Issue: 33
• Pages: 5776-5779
• Publication date: 2010-08-01
• DOI: 10.1002/anie.201001298
• EISSN: 1521-3773
• ISSN: 1433-7851
• Language: English
• Keywords: Electron Transport; Fluorescence Resonance Energy Transfer; Azurin; Immobilized Proteins; Fluorescent Dyes; Thermodynamics; Electrochemical Techniques; Kinetics