Ion mobility mass spectrometry of peptide ions: effects of drift gas and calibration strategies.

Journal article

One difficulty in using ion mobility (IM) mass spectrometry (MS) to improve the specificity of peptide ion assignments is that IM separations are performed using a range of pressures, gas compositions, temperatures, and modes of separation, which makes it challenging to rapidly extract accurate shape parameters. We report collision cross section values (Ω) in both He and N(2) gases for 113 peptide ions determined directly from drift times measured in a low-pressure, ambient temperature drift ...

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Authors: Bush, M; Campuzano, I; Robinson, C

• Publication status: Published
• Journal: Analytical chemistry
• Volume: 84
• Issue: 16
• Pages: 7124-7130
• Publication date: 2012-08-10
• DOI: 10.1021/ac3014498
• EISSN: 1520-6882
• ISSN: 0003-2700
• Language: English
• Keywords: Pressure; Proteomics; Peptides; Temperature; Cattle; Nitrogen; Mass Spectrometry; Helium; Calibration; Amino Acid Sequence; Animals; Molecular Sequence Data