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Journal article

Detection of CFTR transgene mRNA expression in respiratory epithelium isolated from the murine nasal cavity.

Abstract:

BACKGROUND: When assessing the efficacy of gene transfer agents (GTAs) for cystic fibrosis (CF) gene therapy, we routinely evaluate gene transfer in the mouse nose and measure transfection efficiency by assessing transgene-specific mRNA using the real-time (TaqMan) quantitative reverse transcriptase-polymerase chain reaction. TaqMan is traditionally used to quantify expression in whole tissue homogenates, which in the nose would contain many cells types, including respiratory and olfactory ep...

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Publication status:
Published

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Publisher copy:
10.1002/jgm.1413

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Journal:
The journal of gene medicine
Volume:
12
Issue:
1
Pages:
55-63
Publication date:
2010-01-05
DOI:
EISSN:
1521-2254
ISSN:
1099-498X
URN:
uuid:1030af71-8f16-47fb-8049-bb34b65dbf3e
Source identifiers:
246551
Local pid:
pubs:246551

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