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DNA gyrase stimulates transcription.

Abstract:

The nuclear DNA of HeLa cells can now be isolated unbroken and supercoiled. Using DNA gyrase and the untwisting enzyme, we have prepared an allomorphic series of templates derived from this nuclear DNA, and also from the circular DNA of the bacterial virus, PM2. We have then transcribed these templates using 2 different RNA polymerases--from wheat germ and Escherichia coli. Relaxed DNA is transcribed slowly by both polymerases. Supertwisting the naturally-supercoiled templates with gyrase sli...

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Publication status:
Published

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Institution:
University of Oxford
Department:
Oxford, MSD, Pathology Dunn School
Role:
Author
Journal:
Nucleic acids research
Volume:
8
Issue:
4
Pages:
845-854
Publication date:
1980-02-05
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:1009100c-209a-43d2-a093-7ce001fb5d77
Source identifiers:
15643
Local pid:
pubs:15643

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