Differential regulation of tumour necrosis factor receptors (TNF-R) by IL-4; upregulation of P55 and P75 TNF-R on synovial joint mononuclear cells.

Cope, A; Gibbons, D; Aderka, D; Foxwell, B; Wallach, D; Maini, R; Feldmann, M; Brennan, F

Journal article

Differential regulation of tumour necrosis factor receptors (TNF-R) by IL-4; upregulation of P55 and P75 TNF-R on synovial joint mononuclear cells.

Abstract:: Interleukin 4 (IL-4) has previously been shown to downregulate the production of proinflammatory cytokines such as TNF-alpha, and hence has been considered to be a potential anti-inflammatory agent. In this study we have investigated the effects of IL-4 on the expression of both p55 and p75 TNF receptors (TNF-R) by flow cytometry and radioligand binding analyses and demonstrate that IL-4 downregulates both p55 and p75 TNF-R on HeLa and Jijoye cell lines in a dose dependent manner. IL-4 reduced the number of p55 TNF-R on HeLa cells from 6400 (Kd 5.1 nM) to 3900 (Kd 3.7 nM), and p75 TNF-R on Jijoye cells from 4800 (Kd 1.6 nM) to 3250 (Kd 1.5 nM). However, different effects were observed on peripheral blood mononuclear cells (PBMC). IL-4 inhibited the increase in p55 and p75 TNF-R on PBMC following adherence, whereas IL-4 upregulated p75 TNF-R expressed on PHA induced T cell blasts. To assess further the possible anti-inflammatory properties of IL-4, we studied its effects on synovial joint mononuclear cell cultures from 15 patients with inflammatory synovitis. In contrast to the differential effects of IL-4 on monocytes and T cells, IL-4 upregulated both p55 (P < 0.05) and p75 TNF-R (P < 0.005) on synovial joint cells in culture. IL-4 treatment caused a small decrease in levels of bioactive TNF-alpha in RA synovial culture supernatants, together with an increase in soluble p75 TNF-R levels although differences were not significant.(ABSTRACT TRUNCATED AT 250 WORDS)

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APA Style

Cope, A., Gibbons, D., Aderka, D., Foxwell, B., Wallach, D., Maini, R., Feldmann, M., & Brennan, F. (1993). Differential regulation of tumour necrosis factor receptors (TNF-R) by IL-4; upregulation of P55 and P75 TNF-R on synovial joint mononuclear cells. Cytokine, 5(3), 205–212.

MLA Style

Cope, A., et al. “Differential Regulation of Tumour Necrosis Factor Receptors (TNF-R) by IL-4; Upregulation of P55 and P75 TNF-R on Synovial Joint Mononuclear Cells.” Cytokine, vol. 5, no. 3, 1993, pp. 205–12.

Chicago Style

Cope, A, D Gibbons, D Aderka, B Foxwell, D Wallach, R Maini, M Feldmann, and F Brennan. 1993. “Differential Regulation of Tumour Necrosis Factor Receptors (TNF-R) by IL-4; Upregulation of P55 and P75 TNF-R on Synovial Joint Mononuclear Cells.” Cytokine 5 (3): 205–12.
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