APE1 is the major 3'-phosphoglycolate activity in human cell extracts.

Journal article

DNA strand breaks containing 3'-phosphoglycolate (3'-PG) ends are the major lesions induced by ionizing radiation. The repair of this lesion is not completely understood and several activities are thought to be involved in processing of 3'-PG ends. In this study we examined activities in human whole cell extracts (WCE) responsible for removal of 3'-PG. Using a radiolabelled oligonucleotide containing a single nucleotide gap with internal 5'-phosphate and 3'-PG ends, we demonstrate that the major 3'-PG activity in human WCE is Mg2+ dependent and that this activity co-purifies with AP endonuclease 1 (APE1) over phosphocellulose and gel filtration chromatography. Furthermore, immunodepletion of APE1 from active gel filtration fractions using APE1 specific antibodies reveals that the major activity against 3'-PG in human WCE is APE1.

Authors: Parsons, J; Dianova, I; Dianov, G

• Publication status: Published
• Journal: Nucleic acids research
• Volume: 32
• Issue: 12
• Pages: 3531-3536
• Publication date: 2004-01-01
• DOI: 10.1093/nar/gkh676
• EISSN: 1362-4962
• ISSN: 0305-1048
• Language: English
• Keywords: DNA Repair; Cell Extracts; Humans; DNA-(Apurinic or Apyrimidinic Site) Lyase; Precipitin Tests; Hela Cells; Glycolates; Oligonucleotides