DNA triple helix stabilisation by covalent attachment of a triplex-specific ligand.

Journal article

We have prepared oligonucleotides with a naphthylquinoline triplex-binding ligand covalently tethered to the 5'-end and have used UV-melting and DNase I footprinting to examine the stability of intra- and inter-molecular triplexes containing this modification. We find that covalent attachment of the ligand increases the melting temperature of intramolecular 6-mer triplexes by about 14 K, and increases the binding of 9-mer oligonucleotides to their duplex target sites by about 60-fold.

Authors: Keppler, MD; McKeen, C; Zegrocka, O; Strekowski, L; Brown, T

• Publication status: Published
• Journal: Biochimica et biophysica acta
• Volume: 1447
• Issue: 2-3
• Pages: 137-145
• Publication date: 1999-10-01
• DOI: 10.1016/s0167-4781(99)00114-1
• ISSN: 0006-3002
• Language: English
• Keywords: DNA; Quinolines; DNA Footprinting; Ligands; Nucleic Acid Conformation; Animals; Deoxyribonuclease I; Cattle