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123I-Interleukin-2: biochemical characterization and in vivo use for imaging autoimmune diseases.

Abstract:

We describe in detail the labelling of interleukin-2 with I ( I-IL2), its biochemical characterization, the binding assay and its use for the detection of tissues infiltrated with mononuclear cells. Human recombinant IL2 was labelled using an enzymatic method and its biochemical characterization was performed using high performance liquid chromatography (HPLC) analysis of cyanogen bromide-cleaved protein. biological and binding assays were performed on CTLL-2 cell line and on activated periph...

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Publication status:
Published

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Authors


Signore, A More by this author
Picarelli, A More by this author
Annovazzi, A More by this author
Britton, KE More by this author
Grossman, AB More by this author
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Journal:
Nuclear medicine communications
Volume:
24
Issue:
3
Pages:
305-316
Publication date:
2003-03-05
DOI:
EISSN:
1473-5628
ISSN:
0143-3636
URN:
uuid:0f7fc6ee-b4e4-439e-aec4-5c4f9c87d5e0
Source identifiers:
138727
Local pid:
pubs:138727

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