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Synapto-pHluorins: Genetically encoded reporters of synaptic transmission

Abstract:

pHluorins are pH-sensitive mutants of green fluorescent protein (GFP). Attached to proteins with defined cellular locations or itineraries, pHluorins report subcellular pH as well as protein transport between compartments of differing pH. Key applications in neurobiology include the optical detection of neurotransmitter release with synapto-pHluorins and their derivatives, as well as measurements of neurotransmitter receptor trafficking. This article describes the properties and uses of synap...

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Publisher copy:
10.1101/pdb.ip067827

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Journal:
Cold Spring Harbor Protocols
Volume:
7
Issue:
2
Pages:
213-217
Publication date:
2012-02-05
DOI:
EISSN:
1559-6095
ISSN:
1940-3402
URN:
uuid:0f4dda98-e901-494a-b7c9-73753a24083c
Source identifiers:
313840
Local pid:
pubs:313840
Language:
English

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