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A simplified method of generating transgenic Xenopus

Abstract:
Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.
Publication status:
Published
Peer review status:
Peer reviewed

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Publisher copy:
10.1093/nar/28.4.e12

Authors


More by this author
Institution:
University of Oxford
Department:
Oxford, MSD, Physiology Anatomy and Genetics
Latinkic, B More by this author
Medical Research Council More from this funder
British Heart Foundation More from this funder
Publisher:
Oxford University Press Publisher's website
Journal:
Nucleic Acids Research Journal website
Volume:
28
Issue:
4
Pages:
12e-12
Publication date:
2000
DOI:
EISSN:
1362-4962
ISSN:
0305-1048
URN:
uuid:0ef4190f-0d8b-4c6d-bb7f-0da4fcc0ca90
Source identifiers:
573475
Local pid:
pubs:573475

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