Journal article icon

Journal article

Identification of serotype in culture negative pneumococcal meningitis using sequential multiplex PCR: implication for surveillance and vaccine design.

Abstract:

BACKGROUND: PCR-based serotyping of Streptococcus pneumoniae has been proposed as a simpler approach than conventional methods, but has not been applied to strains in Asia where serotypes are diverse and different from other part of the world. Furthermore, PCR has not been used to determine serotype distribution in culture-negative meningitis cases. METHODOLOGY: Thirty six serotype-specific primers, 7 newly designed and 29 previously published, were arranged in 7 multiplex PCR sets, each in ...

Expand abstract
Publication status:
Published
Peer review status:
Peer reviewed
Version:
Publisher's version

Actions


Access Document


Files:
Publisher copy:
10.1371/journal.pone.0003576

Authors


Expand authors...
Wellcome Trust-Burroughs Wellcome Fund Infectious Disease Initiative More from this funder
GAVI's PneumoADIP More from this funder
Johns Hopkins University More from this funder
Dhaka Shishu Hospital More from this funder
Publisher:
Public Library of Science Publisher's website
Journal:
PloS one Journal website
Volume:
3
Issue:
10
Pages:
e3576
Publication date:
2008-01-01
DOI:
EISSN:
1932-6203
ISSN:
1932-6203
URN:
uuid:0ebdde74-3675-4323-9191-d685632b32d7
Source identifiers:
66785
Local pid:
pubs:66785

Terms of use


Metrics


Views and Downloads






If you are the owner of this record, you can report an update to it here: Report update to this record

TO TOP