Journal article
Identification of serotype in culture negative pneumococcal meningitis using sequential multiplex PCR: implication for surveillance and vaccine design.
- Abstract:
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BACKGROUND: PCR-based serotyping of Streptococcus pneumoniae has been proposed as a simpler approach than conventional methods, but has not been applied to strains in Asia where serotypes are diverse and different from other part of the world. Furthermore, PCR has not been used to determine serotype distribution in culture-negative meningitis cases. METHODOLOGY: Thirty six serotype-specific primers, 7 newly designed and 29 previously published, were arranged in 7 multiplex PCR sets, each in ...
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- Publication status:
- Published
- Peer review status:
- Peer reviewed
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(Version of record, pdf, 345.0KB)
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- Publisher copy:
- 10.1371/journal.pone.0003576
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Authors
Funding
GAVI's PneumoADIP
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Wellcome Trust-Burroughs Wellcome Fund Infectious Disease Initiative
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Johns Hopkins University
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Dhaka Shishu Hospital
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Bibliographic Details
- Publisher:
- Public Library of Science Publisher's website
- Journal:
- PloS one Journal website
- Volume:
- 3
- Issue:
- 10
- Pages:
- e3576
- Publication date:
- 2008-01-01
- DOI:
- EISSN:
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1932-6203
- ISSN:
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1932-6203
- Source identifiers:
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66785
Item Description
- Language:
- English
- Keywords:
- Pubs id:
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pubs:66785
- UUID:
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uuid:0ebdde74-3675-4323-9191-d685632b32d7
- Local pid:
- pubs:66785
- Deposit date:
- 2012-12-19
Terms of use
- Copyright holder:
- Saha et al
- Copyright date:
- 2008
- Notes:
- Copyright 2008 Saha et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
- Licence:
- CC Attribution (CC BY)
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