Circulating prothymosin alpha and immunoglobulin G3 in acute rheumatic fever and rheumatic heart disease: A case-control study

Journal article

IntroductionThe lack of specific diagnostic tests for acute rheumatic fever (ARF) and its consequence rheumatic heart disease (RHD) is a key barrier to effective control of these diseases in low resource settings.MethodsWe used immunoassays to evaluate blood prothymosin alpha (PTA) and immunoglobulin G3 (IgG3) in patients with ARF, RHD and controls using serum samples from Pakistan and plasma samples from Uganda.ResultsAcross both sets of samples, we found total IgG3 was significantly elevated in definite ARF (n = 24) compared to controls (n = 36), possible ARF (n = 15) and chronic RHD (n = 11). Whether measured in serum or plasma, PTA levels were similar across these groups.DiscussionOur findings conflict with a previous report that found PTA was elevated in RHD compared to healthy controls. In contrast, while less marked than before, we found IgG3 was elevated in ARF, extending this observation to settings where the impact of RHD is greatest.

Authors: Afshan, G; Tsui, N; Javed, H; Kazmi, T; Ndagire, E

• Publication status: Published
• Peer review status: Peer reviewed
• Publisher: Elsevier
• Journal: American Heart Journal Plus: Cardiology Research and Practice
• Volume: 59
• Pages: 100630
• Publication date: 2025-09-29
• Acceptance date: 2025-09-26
• DOI: 10.1016/j.ahjo.2025.100630
• EISSN: 2666-6022
• ISSN: 2666-6022
• Pmid: 41126870
• Language: English
• Keywords: Immunoglobulin-G3; Acute Rheumatic Fever; Streptococcus pyogenes; Prothymosin-alpha; Biomarkers; Rheumatic heart disease