The reliable discrimination of mutations, single nucleotide polymorphisms (SNPs), and other differences in genomic sequence is an essential part of DNA diagnostics and forensics. It is commonly achieved using fluorescently labeled DNA probes and thermal gradients to distinguish between the matched and mismatched DNA. Here, we describe a novel method that uses surface enhanced (resonance) Raman spectroscopy (SER(R)S) to follow denaturation of dsDNA attached to a structured gold surface. This d...Expand abstract
- Publication status:
- Publisher copy:
- Copyright date:
SERS-melting: a new method for discriminating mutations in DNA sequences.
If you are the owner of this record, you can report an update to it here: Report update to this record